Venkatanaidu Karri

INTEGRATED IN VITRO – OMICS BASED APPROACH TO ASSESS THE HEAVY METALS AND THEIR BINARY MIXTURES TOXICITY IN HIPPOCAMPAL HT-22 CELL LINE

Environmental exposures are virtually always to complex metal mixtures. Current toxicological studies dictate a largely metal-by-metal evaluation of exposures and risks in the brain, which fails to adequately address potential interactions of metals in the hippocampus region of the brain. The scope of our study is to prove the single metal (Pb, Cd, As, and MeHg) and their binary mixtures chronic toxicity impact in hippocampal cells by using the in vitro and systems biology methodology. This enables to gain a global understanding of the way in which heavy metal single and mixtures exposures response in the hippocampal cells. Gene functional and pathway analyses of proteomic data confirms alterations related to mitochondrial dysfunction, oxidative stress, mRNA splicing, and ubiquitin system dysfunction related neurodegeneration. We concluded from the protein data and pathways, metal mixtures exposure is more hazardous than single metal exposure in HT-22 hippocampal cells.

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